| ORGANOLEPTIC TEST | | |
| Appearance and structure, cross-section surface, consistency, smell, taste, foreign /visible/ impurities | COMECON ST 4710 | |
| | |
| PHYSICO-CHEMICAL AND BIOCHEMICAL TESTS | | |
| Active acidity (рН) | QOP 504-1-FH-26 | |
| Titrable (free acidity) | BDS 4336 item4.9 | |
| Water content/dry matter; Moisture | BDS 11374 item4.1; BDS 15435 item4.8 | |
| Content of volatile | BDS ISO 6496 | |
| Fat content (crude fat) - extractive | BDS EN ISO 11085; BDS 12896; BDS ISO 6492; BDS 11374 item4.4.3.1 | |
| Protein content (protein, crude protein, nitrogen, soluble nitrogen, pepsin-soluble protein) | BDS ISO 1871; BDS 11374 item4.2; BDS ISO 6655; Наредба 44/2002, приложение 6, метод XXIII | |
| Sugars content - lactose | Наредба 44/2002, приложение 6, метод VIII; XI | |
| Carbohydrates content | QOP 504-1-FH-41 | |
| Starch content (starch) | BDS EN ISO 15914; Наредба 44/2002, метод XXI | |
| Crude cellulose content (fibers) | BDS EN ISO 6865; BDS 11374 item4.3 | |
| Crude cellulose content (fibers) - acid detergent fibers (АDF) and acid detergent ligning (ADL) | BDS EN ISO 13906 | |
| Crude cellulose content (fibers) – neutral detergent fibers(aNDF) | BDS EN ISO 16472 | |
| Ash content – crude ash | BDS EN ISO 2171; BDS ISO 5984; BDS 11374 item4.5 | |
| Ash content - insoluble ash | BDS ISO 5985; BDS 11374 item4.5 | |
| Table salt content /sodium chloride | QOP 504-1-FH-03; BDS 11374 item4.10 | |
| Energy value | Наредба 44/2002,Раздел IV | |
| Content of ascorbic acid (Vitamin C) | QOP 504-1-FH-45 | |
| Sodium hydroxide residues content | QOP 504-1-FH-15 | |
| Chlorine residues content | QOP 504-1-FH-27 | |
| Testing for presence of epihydrin aldehyde through Kreis reaction (Fat rancidity) | QOP 504-1-FH-30 | |
| Vegetable fats presence in milk fat through Bélier reaction | QOP 504-1-FH-29 | |
| Determination of peroxide value | BDS 11374 item4.15 | |
| Benzoic acid content | BDS 1554 item III.15 | |
| Potassium sorbate content | BDS 11712 item2 | |
| Sulfur dioxide content (total and free) | BDS EN 1185 | |
| Soy protein content | QOP 504-1-FH-32 | |
| Aluminum content | QOP 504-1-FH-07 | |
| Antimony content | QOP 504-1 ICP/MS-04 | |
| Arsenic content | QOP 504-1-FH-19; BDS EN 15763 | |
| Barium ions content | BDS EN ISO 17294-2 | |
| Boron content | QOP 504-1-FH-58 | |
| Iron content | QOP 504-1-FH-02 | |
| Mercury content | BDS EN 15763 | |
| Iodine content | QOP 504-1-FH-46 | |
| Cadmium content | QOP 504-1-FH-17; BDS EN 15763 | |
| Tin content | QOP 504-1-FH- 57 | |
| Calcium content | QOP 504-1-FH-21; BDS 11374 item4.11.2 | |
| Magnesium content | QOP 504-1-FH-52 | |
| Manganese content | QOP 504-1-FH-04 | |
| Copper content | QOP 504-1-FH-18 | |
| Sodium content | QOP 504-1 ICP/MS-02 | |
| Nickel content | QOP 504-1-FH-24 | |
| Nitrates content | QOP 504-1-FH-08 | |
| Nitrites content | QOP 504-1-FH-05; BDS 1035 | |
| Lead content | QOP 504-1-FH-16; BDS EN 15763 | |
| Selenium content | QOP 504-1-FH-59 | |
| Sulphates content | QOP 504-1-FH-09 | |
| Phosphates content | QOP 504-1-FH-06; BDS ISO 6491; BDS 11374 item4.11.4 | |
| Chromium content | QOP 504-1-FH-25 | |
| Cyanides content | QOP 504-1-FH-53 | |
| Zinc content | QOP 504-1-FH-23 | |
| Aflatoxin В1 content | QOP 504-1-FH-20 | |
| Aflatoxines В1+В2+G1+G2 (total) | QOP 504-1-FH-34 | |
| Aflatoxin M1 content | QOP 504-1-FH-31 | |
| Deoxynivalenol /DON/ content | QOP 504-1-FH-35; BDS EN 15791 | |
| Zearalenone content | QOP 504-1-FH-36 | |
| Ochratoxin content | QOP 504-1-FH-38 | |
| Т2 toxin content | QOP 504-1-FH-39 | |
| Fumonisin content | QOP 504-1-FH-37 | |
| Ammonia content according to Nessler method | BDS 1323 item2.3.5 | |
| Ammonia content according to Eber’s test | BDS 1323 item2.3.6 | |
| Hydrogen sulphide | BDS 1323 item 2.3.8 | |
| Ethanol (ethyl alcohol) content | BDS ISO 2448 | |
| Urea content | BDS EN ISO 14637 | |
| Connective tissue protein (hydroxyproline) content | BDS 14780 | |
| Histamine content | QOP 504-1-FH-33 | |
| Proportional content of ingredients | BDS 7181 item3; BDS 15359 item2 | |
| Net weight determination | BDS 7181 item2; BDS 15359 item1 | |
| Urea content | BDS 11374 item4.12 | |
| Potassium content | BDS EN ISO 17294-2 | |
| Gossypol content (glucoside from cottonseed) | BDS ISO 6866 | |
| Activity of trypsin inhibitors | BDS EN ISO 14902 | |
| Nitrogen-free extract substances /NFES/ content | BDS 11374 item4.6 | |
| Monensin content | BDS 11374 item5.1 | |
| Ethoxyquin/santoquin content | BDS 11374 item5.9 | |
| Presence of organophosphorous pesticides | BDS 11374 item8 | |
| Content of pesticide: Chlororganic Phosphororganic Nitrogen and sulfur-containing | BDS EN ISO 15662 | |
| Sweeteners content: | | |
| - acesulfame К | BDS EN 12856; BDS EN 15911 | |
| - saccharine | BDS EN 12856; BDS EN 15911 | |
| - aspartame | BDS EN 12856; BDS EN 15911 | |
| - cyclamate | BDS EN 12857; BDS EN 15911 | |
| Colorings content: | | |
| Yellow: | | |
| - Sunset Yellow | QOP 504-1-IM-HPLC-01 | |
| - tartrazine | QOP 504-1-IM-HPLC-01 | |
| Red: | | |
| - erythrosine | QOP 504-1-IM-HPLC-01 | |
| - azorubine | QOP 504-1-IM-HPLC-01 | |
| - allura red | QOP 504-1-IM-HPLC-01 | |
| - ponceau | QOP 504-1-IM-HPLC-01 | |
| - amaranth | QOP 504-1-IM-HPLC-01 | |
| Blue | | |
| - indigotine | QOP 504-1-IM-HPLC-01 | |
| - Brillian Blue | QOP 504-1-IM-HPLC-01 | |
| - Patent Blue | QOP 504-1-IM-HPLC-01 | |
| Vitamin content: | | |
| - А (retinol) | BDS EN ISO 14565 | |
| - А (beta-carotine) | BDS EN ISO 14565 | |
| - В1 (thiamine) | QOP 504-1-IM-LC/MS-02 | |
| - В3 (niacin) | QOP 504-1-IM-LC/MS-02 | |
| - В6 (pyridoxine) | QOP 504-1-IM-LC/MS-02 | |
| - В7 (biotin/vitamin Н) | QOP 504-1-IM-LC/MS-02( | |
| - С (ascorbic acid) | BDS EN 14130 | |
| - D (D2 ergocalciferol) | BDS EN 12821 | |
| - D (D3 colecalciferol) | BDS EN 12821 | |
| - Е (tocopherols) | BDS EN ISO 6867 | |
| - К (phytomenadione) | BDS EN 14148 | |
| Content of saturated fatty acids | BDS EN ISO 12966-2+BDS EN ISO 5508 | |
| Content of unsaturated fatty acids | BDS EN ISO 12966-2+BDS EN ISO 5508 | |
| Pharmacological substances residues content: | | |
| Antibiotics (Quinolines) – flumequin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfaguanidin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfadiazine | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfamerazin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfamethoxazole | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfapyridine | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfatiazol | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfacetamide | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – oxytetracycline хидрохлорид | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – tetracycline hydrochloride | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – chlortetracycline hydrochloride | QOP 504-1-IM-LC/MS-01 | |
| MICROBIOLOGICAL TESTS | | |
| Total number of microorganisms: | | |
| Total number of microorganisms, including mesophilic aerobes and facultative anaerobes | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic aerobs non-spore-forming microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic aerobs vegetative forms of spore-forming microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic aerobs spore forms of saprophytic microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic anaerobs microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic anaerobs microorganisms - vegetative forms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Mesophilic anaerobs microorganisms - spore forms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic aerobes and facultative anaerobes microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic aerobs microorganisms - non-spore-forming | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic aerobes microorganisms - vegetative forms of spore-forming | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic aerobes microorganisms - spore forms of saprophytic | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic anaerobs microorganisms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic anaerobs microorganisms - vegetative forms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Thermophilic anaerobs microorganisms - spore forms | BDS ЕN ISO 4833-1; BDS ЕN ISO 4833-2; BDS 1035 item5.9.1-4; BDS 1035 item5.9.6.2; item5.9.6.3; BDS 6877 item2.3 | |
| Antibiotics and Sulfonamides – presence / absence | BDS 1323 item4.3.1 | |
| Bacillus species | BDS EN 15784 | |
| Bifidobacterium species | BDS EN 15785 | |
| Brochothrix thermosphacta | BDS ISO 13722 | |
| Vibrios (other than Vibrio parahaemolyticus and Vibrio cholerae) | ISO/TS 21872-2 | |
| Vibrio parahaemolyticus; Vibrio cholerae | ISO/TS 21872-1 | |
| Water activity | ISO 21807 | |
| Yeast | BDS ISO 21527-1; -2 | |
| Escherichia coli | ISO 16649-2; ISO 7251 item9.1; item9.2; | |
| Escherichia coli O157 | BDS EN ISO 16654 | |
| Enterobacter (cronobacter) sakazakii | ISO/TS 22964 | |
| Enterobacteria, including Proteus, Klebsiella, Citrobacter | ISO 21528-1 item9.2; item9.3; ISO 21528-2 | |
| Enterococus faecium | BDS EN 15788 | |
| Yersinia enterocolitica | BDS EN ISO 10273 | |
| Campylobacter spp. | ISO 10272-1; BDS ISO/TS 10272-2 | |
| Clostridia perfringens | ISO 7937 | |
| Clostridia Sulfite-reducing | ISO 15213 | |
| Coagulase-positive staphylococci | BDS EN ISO 6888-1+A1; -2+A1; -3+AC item9.1; item9.2 | |
| Coliforms | ISO 4831 item9.1; item9.2; ISO 4832 | |
| Lactobacillus species | BDS EN 15787 | |
| Legionella | BDS EN ISO 11731-2 | |
| Leuconostoc | COMECON ST 5806 | |
| Lipolytic microorganisms | BDS 12334 item7 | |
| Listeria monocytogenes | BDS EN ISO 11290-1+А1; -2+А1 | |
| Mesophilic lactic bacteria | BDS ISO 15214 | |
| Acetobacter microorganisms | BDS 15143 item5.1 | |
| Pediococcus species | BDS EN 15786 | |
| Moulds | BDS ISO 21527-1; -2 | |
| Probiotics (saccharomyces cerevisiae) | BDS EN 15789 | |
| Proteolytic microorganisms | BDS 3434 item10.3 | |
| Pseudomonas aeruginosa | BDS EN ISO 13720 | |
| Psychrotrophic microorganisms | BDS ISO 17410 | |
| Salmonella species | BDS ЕN ISO 6579; SD СЕN ISO/ TS 6579-2 | |
| Streptococci, including Streptococcus thermophilus | BDS 1323 item5.4.5.2; BDS 14593 item6.9; BDS ISO 9232; BDS ISO 7889 | |
| Thermostatic test | BDS 1035 item3.4; BDS 6877 item2.1 | |
| Halophilic (salt-tolerant) microorganisms | BDS 3434 item10.4 | |
| Shigella species | BDS EN ISO 21567 | |
| Anthrax | BDS 1323 item5.4.3 | |
| Contaminating microorganisms | ISO 13559(1) | |
| GENETICALLY MODIFIED ORGANISMS | | |
| Determination of presence of genetically modified organisms – 35S | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |
| Determination of presence of genetically modified organisms – FMV | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |
| Determination of presence of genetically modified organisms – NOS terminator | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |